Explain Podcast

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Date Title & Description Contributors

  Episode 5: Illumina Sequencing Part II

Glued DNA bridges and rainbow clusters Chapters: 00:43-Flowcells 03:40-DNA glued to glass 09:00-Bridge amplification 15:50-Cluster density and patterned Flowcells 28:30-Illumina SBS summary 30:30-Limits 33:00-Multiplexing and Demultiplexing - enseq...

  Episode 5: Illumina Sequencing Part I

Colour mixing in PHRED space Chapters: 00:00 Nucleotides level 2 09:00 Cycles 12:00 Not-so-wobbly mutations 15:30 Reducing colourspace 22:30 Cutting connections 28:14 Costs and PHRED - ClevaLabs on Illumina sequencing: https://www.youtube.com/watch...

  Special Episode 2: How Sequencing is used

Iuliia killed the stripey cat Chapters: 00:01:00 Intro 00:04:44 Stripes 00:11:40 Sanger with colors 00:17:20 CATCATCAT... 00:33:20 Planes 00:38:15 Genome assembly 00:40:00 Transcriptomics 01:01:00 Hi-C Why women are stripey: https://www.youtube.co...

  Episode 4: Ion Torrent Sequencing

H+ ions and where we can find them Chapters: 00:00 Intro 04:26 pH 08:04 DNA Polymerase reaction with H+ 12:30 Nucleotides 16:30 Homopolymer problem, but different 19:30 PCR is mean to some bases 24:30 AmpliSeq 33:40 Today’s status 38:58 cfDNA 40:00 P...

  Episode 3: SOLiD Sequencing

If ligation is more your thing - Special Operations explaining SOLiD Sequencing: https://www.youtube.com/watch?v=YLT-DUeaLms - Writeup by the Apollo Institute on SOLiD Sequencing: https://apollo-institute.org/solid-sequencing/ - Quick run-through vi...

  Special Episode 1: Kitchen Genomics - DIY DNA Isolation

Iuliia and Daniel have fun with salt water and alcohol Chapters: 00:00:00 DIY DNA isolation 00:15:38 Directed evolution of Enzymes DNA isolation in your kitchen - Experiment during the episode: https://www.instructables.com/How-to-Extract-Your-Own-...

  Episode 2: Pyrosequencing

Daniel and Iuliia are confused about fireflies - Scientific american on how fireflies create light: https://www.scientificamerican.com/article/how-and-why-do-fireflies/ - A small breakdown of a typical Pyrosequencing workflow: https://www.news-medica...

  Episode 1: Basics - from DNA to Sanger sequencing

Iuliia is confused about RNA and Daniel doesn’t know the size of a DNA nucleotide Chapters: 00:00-Intro 02:00-DNA 08:27-Enzymes 13:05-RNAs, tRNAs 16:18-mRNAs, µRNAs, lncRNAs, circRNAs 21:35-Wobble 23:30-PCR 33:20-Sanger sequencing I 36:30-Gel electrop...


The Explain Podcast hosts are bioinformatician Daniel and scientific coordinator Iuliia. We aim to EXPLAIN key next generation sequencing techniques and how these techniques contribute to our everyday life.The Podcast Explain is for everyone interested...