Explain Podcast
The Podcast Explain is for everyone interested in DNA/RNA sequencing or technology in general. As Iuliia doesn't have a solid background in sequencing, she keeps asking "simple" - even silly at times - questions and Daniel gives his best to make us understand.
- Website
- https://ngs-kn.de/explain-podcast/
- Description
- The Podcast Explain is for everyone interested in DNA/RNA sequencing or technology in general. As Iuliia doesn't have a solid background in sequencing, she keeps asking "simple" - even silly at times - questions and Daniel gives his best to make us understand.
- Language
- 🇬🇧 English
- last modified
- 2024-05-13 03:20
- last episode published
- 2024-03-22 14:14
- publication frequency
- 23.93 days
- Contributors
- explain_podcast
- Author
- explain_podcast
- Owner
- Explicit
- false
- Number of Episodes
- 13
- Rss-Feeds
- Detail page
- Categories
- Science Natural Sciences
Recommendations
Episodes
| Date | Title & Description | Contributors |
|---|---|---|
| 2024-03-22 | Chapters: 08:00 Gambling 15:00 Bubbles 30:00 Holes 57:30 One more thing 1:07:00 Finale 1:08:00 Finale of season 1! Eric Chow explaining single cell sequencing: https://www.youtube.com/watch?v=k9VFNLLQP8c Overview of things to consider: https://www.... | |
| 2024-02-09 | Getting the most out of Machines Chapters: 04:30 PhiX 14:30 low complexity 19:30 UMIs 32:10 FastQC 43:00 MultiQC 56:40 PycoQC PhiX concentrations for loading a validation run: https://knowledge.illumina.com/instrumentation/general/instrumentation-gen... | |
| 2024-01-16 | Pushing spaghetti through holes Chapers: 00:00 Intro 11:15 Measuring Ions 17:00 Quality 23:00 Duplex 24:30 MinION 25:15 PromethION 28:20 Methylation 29:00 Direct RNA sequencing 38:25 Basecalling 46:00 Basecalling bias 53:50 Sequencing proteins - Nano... | |
| 2023-12-21 | Zipping rounds in zeptoliters Chapters: 00:00 Intro 07:00 Laser 09:00 Zeptoliters 13:30 Polymerase speed 19:00 Error rate 22:00 SMRT Cell 25:45 ccs 30:00 Fragment size 34:30 Megaruptor 38:20 Data flow 47:00 SVs 53:00 MAS Seq - Starter: https://www.n... | |
| 2023-12-01 | Glued DNA bridges and rainbow clusters Chapters: 00:43-Flowcells 03:40-DNA glued to glass 09:00-Bridge amplification 15:50-Cluster density and patterned Flowcells 28:30-Illumina SBS summary 30:30-Limits 33:00-Multiplexing and Demultiplexing - enseq... | |
| 2023-11-13 | Colour mixing in PHRED space Chapters: 00:00 Nucleotides level 2 09:00 Cycles 12:00 Not-so-wobbly mutations 15:30 Reducing colourspace 22:30 Cutting connections 28:14 Costs and PHRED - ClevaLabs on Illumina sequencing: https://www.youtube.com/watch... | |
| 2023-10-20 | Iuliia killed the stripey cat Chapters: 00:01:00 Intro 00:04:44 Stripes 00:11:40 Sanger with colors 00:17:20 CATCATCAT... 00:33:20 Planes 00:38:15 Genome assembly 00:40:00 Transcriptomics 01:01:00 Hi-C Why women are stripey: https://www.youtube.co... | |
| 2023-09-22 | H+ ions and where we can find them Chapters: 00:00 Intro 04:26 pH 08:04 DNA Polymerase reaction with H+ 12:30 Nucleotides 16:30 Homopolymer problem, but different 19:30 PCR is mean to some bases 24:30 AmpliSeq 33:40 Today’s status 38:58 cfDNA 40:00 P... | |
| 2023-08-22 | If ligation is more your thing - Special Operations explaining SOLiD Sequencing: https://www.youtube.com/watch?v=YLT-DUeaLms - Writeup by the Apollo Institute on SOLiD Sequencing: https://apollo-institute.org/solid-sequencing/ - Quick run-through vi... | |
| 2023-08-04 | Iuliia and Daniel have fun with salt water and alcohol Chapters: 00:00:00 DIY DNA isolation 00:15:38 Directed evolution of Enzymes DNA isolation in your kitchen - Experiment during the episode: https://www.instructables.com/How-to-Extract-Your-Own-... |