The Podcast Explain is for everyone interested in DNA/RNA sequencing or technology in general. As Iuliia doesn't have a solid background in sequencing, she keeps asking "simple" - even silly at times - questions and Daniel gives his best to make us understand.
Date | Title & Description | Contributors |
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2024-08-01 | Mixing up long and cheap Chapters: 00:00:00 Intro 00:03:30 Florians history 00:13:50 Q&A Ressources for Bioinformatics 00:27:00 Long-read-tools.org 00:35:42 ONT || PacBio 00:57:00 Storage 01:09:00 Outro Up-to-date, raw prices: https://albertvilel... | |
2024-07-02 | Rolling in circles to make DNA yarns Chapters: 59.091494 288.060099 Bioinformatics Upper Crust Tools 113.762865 288.060099 STAR 288.060099 558.377366 MGI 558.871056 698.232632 Advantages of the DNBs/-yarns 699.178454 874.617258 Using basic DNA charact... | |
2024-05-27 | Who needs all bases anyway? Chapters: 03:00 Phasing 08:50 not Sequencing, really 14:00 Bionano Saphyr Chip 26:00 Nabsys - going electric 29:30 tools available 39:00 Iuliia does QuickGen: Transposons! Links: Small explainer by CAP: https://www.cap.org... | |
2024-03-22 | Chapters: 08:00 Gambling 15:00 Bubbles 30:00 Holes 57:30 One more thing 1:07:00 Finale 1:08:00 Finale of season 1! Eric Chow explaining single cell sequencing: https://www.youtube.com/watch?v=k9VFNLLQP8c Overview of things to consider: https://www.... | |
2024-02-09 | Getting the most out of Machines Chapters: 04:30 PhiX 14:30 low complexity 19:30 UMIs 32:10 FastQC 43:00 MultiQC 56:40 PycoQC PhiX concentrations for loading a validation run: https://knowledge.illumina.com/instrumentation/general/instrumentation-gen... | |
2024-01-16 | Pushing spaghetti through holes Chapers: 00:00 Intro 11:15 Measuring Ions 17:00 Quality 23:00 Duplex 24:30 MinION 25:15 PromethION 28:20 Methylation 29:00 Direct RNA sequencing 38:25 Basecalling 46:00 Basecalling bias 53:50 Sequencing proteins - Nano... | |
2023-12-21 | Zipping rounds in zeptoliters Chapters: 00:00 Intro 07:00 Laser 09:00 Zeptoliters 13:30 Polymerase speed 19:00 Error rate 22:00 SMRT Cell 25:45 ccs 30:00 Fragment size 34:30 Megaruptor 38:20 Data flow 47:00 SVs 53:00 MAS Seq - Starter: https://www.n... | |
2023-12-01 | Glued DNA bridges and rainbow clusters Chapters: 00:43-Flowcells 03:40-DNA glued to glass 09:00-Bridge amplification 15:50-Cluster density and patterned Flowcells 28:30-Illumina SBS summary 30:30-Limits 33:00-Multiplexing and Demultiplexing - enseq... | |
2023-11-13 | Colour mixing in PHRED space Chapters: 00:00 Nucleotides level 2 09:00 Cycles 12:00 Not-so-wobbly mutations 15:30 Reducing colourspace 22:30 Cutting connections 28:14 Costs and PHRED - ClevaLabs on Illumina sequencing: https://www.youtube.com/watch... | |
2023-10-20 | Iuliia killed the stripey cat Chapters: 00:01:00 Intro 00:04:44 Stripes 00:11:40 Sanger with colors 00:17:20 CATCATCAT... 00:33:20 Planes 00:38:15 Genome assembly 00:40:00 Transcriptomics 01:01:00 Hi-C Why women are stripey: https://www.youtube.co... |