Mentors at Your Benchside   /     Cryo-electron Tomography for Cell Biology

Description

In this webinar, you will learn: - The complete workflow for in situ cryo-electron tomography - How subtomogram averaging within the cell yields native-state structures of macromolecular complexes (e.g., the asymmetric and dilated nuclear pore of algae) - How mapping these structures back into the native cellular environment reveals new molecular interactions that are only accessible by this technique (e.g., the binding of cargo to COPI-coated Golgi membranes and the tethering of proteasomes to the nuclear pore). Cryo-electron tomography can visualize macromolecular structures in situ, inside the cell. Vitreous frozen cells are first thinned with a focused ion beam and then imaged in three dimensions using a transmission electron microscope. This transformative method has the power to revolutionize our understanding of cell biology, revealing native cellular architecture with molecular clarity.

Subtitle
Duration
00:58:51
Publishing date
2019-04-11 13:00
Link
https://bitesize.hipcast.com/deluge/bitesize-20190412173452-9617.mp3
Contributors
Enclosures
https://bitesize.hipcast.com/deluge/bitesize-20190412173452-9617.mp3
audio/mpeg